Search results for "Xanthurenic acid"
showing 3 items of 3 documents
Separation by FPLC chromatofocusing of UDP-glucosyltransferases from three developmental stages of Drosophila melanogaster.
1997
Variation of UDP-glucosyltransferase activity, during Drosophila melanogaster development, was analyzed. The endogenous metabolite xanthurenic acid and the xenobiotic compounds 1-naphthol and 2-naphthol were used as substrates. Developmentally regulated differences were observed for the three substrates, suggesting the presence of UDP-glucosyltransferase isoenzymes. This was further confirmed by FPLC chromatofocusing on a Mono P column: seven peaks of UDP-glucosyltransferase activity (pHs: ≥6.3, 5.8, 5.5, 4.9, 4.5, 4.2, ≤4.0) with either single or overlapping substrate specificity were detected. A single xanthurenic acid:UDP-glucosyltransferase activity (pl 5.8) was found throughout develop…
Phenobarbital Induction of UDP-glucosyltransferase Activity in Drosophila melanogaster Meigen
2000
The inducibility of UDP-glucosyltransferase activities towards the exogenous substrates 1-naphthol and 2-naphthol and the endogenous metabolite xanthurenic acid was demonstrated in Drosophila melanogaster Meigen larvae and adults using phenobarbital as an inducer. In adults, a 3.5-fold increase of glucosyltransferase activity toward xanthurenic acid and a 2.0-fold increase of the activity toward exogenous substrates (1-naphthol and 2-naphthol) was found. In larvae, maximum induction of all three UDP-glucosyltransferase activities (2.5-fold and 1.5-fold increase of the activity toward the exogenous and endogenous substrates, respectively) was achieved when insects, reared on solid medium, we…
Biosynthesis of xanthurenic acid 8-O-beta-D-glucoside in Drosophila. Characterization of the xanthurenic acid:UDP-glucosyltransferase activity.
1990
Xanthurenic acid 8-glucoside is a side metabolite of the tryptophan-xanthommatin pathway in Drosophila. From 3-hydroxykynurenine, two biosynthetic pathways can be envisaged, one via xanthurenic acid, and another via 3-O-glucoside of 3-hydroxykynurenine. In this report evidence is presented to show that the synthesis takes place via xanthurenic acid. (a) We have demonstrated that the Drosophila melanogaster vermilion purple mutant (unable to synthesize 3-hydroxykynurenine) synthesizes xanthurenic acid 8-glucoside when fed with xanthurenic acid; and (b) the activities required for its synthesis via xanthurenic acid have been found (3-hydroxykynurenine transaminase and xanthurenic acid:UDP-glu…